Assay Method Information

Assay Name:  Fluorescence polarization-based biochemical high throughput dose response assay for activators of the Protein Kinase A-R2B (PKA-R2B) complex
Description:  Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: The Scripps Research Institute, TSRI Assay Provider: Susan Taylor, University of California, San Diego (UCSD) Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: R01 GM34921 Grant Proposal PI: Susan Taylor, University of California, San Diego (UCSD) External Assay ID: PKAR2B_ACT_FP_1536_3XEC50 DRUN Name: Fluorescence polarization-based biochemical high throughput dose response assay for activators of the Protein Kinase A-R2B (PKA-R2B) complex. Description: Protein phosphorylation is one of the most important mechanisms for regulation in mammalian cells, and cAMP is an ancient second messenger signaling molecule. Protein kinase A (PKA) is a ubiquitous cAMP-dependent serine/threonine protein kinase that phosphorylates intracellular protein substrates in response to the secondary messenger, adenosine 3',5'-cyclic monophosphate (cAMP
Affinity data for this assay
 

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