| Assay Method Information | |
| | Dose response confirmation uHTS hits for MazEF TA System activators via a fluorescence-based single-stranded RNase assay counterscreen |
| Description: | Data Source: Sanford-Burnham Center for Chemical Genomics (SBCCG) Source Affiliation: Sanford-Burnham Medical Research Institute (SBMRI, San Diego, CA) Network: NIH Molecular Libraries Probe Production Centers Network (MLPCN) Grant Number: 2R01 GM068385-06 Assay Provider: Dr. Paul Hergenrother, University of Illinois, Urbana, IL The assay was developed and performed in dose-response as a counter assay for the primary assay originally identified as "identification of MazEF TA System activators via a fluorescence-based single-stranded RNase assay", AID 504720. The goal of the assay is to identify compounds that exhibit an increase in fluorescence over the timecourse of the assay in the absence of the MazEF TA system. The counter dose-response assay described here will enable us to filter fluorescent compounds and readily identify small molecules that activate RNase-based toxins. A fluorophore and a quencher are on the opposite ends of an oligonucleotide substrate. When the RNase (i |
| Affinity data for this assay | |
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