| Assay Method Information | |
| | ChEMBL_2020021 (CHEMBL4673834) |
| Description: | Inhibition of recombinant wild-type human COMT expressed in Escherichia coli BL21 using 4-nitrocatechol-Alexa488 as substrate in presence of cofactor SAM preincubated for 1 min followed by substrate addition and measured every 60 sec up to 180 times by fluorescence based microtiter plate reader analysis |
| Affinity data for this assay | |
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