| Assay Method Information | |
| | ChEMBL_2100444 (CHEMBL4808840) |
| Description: | Inhibition of human PDE5A1 catalytic domain (Glu535 to Gln860 residues) expressed in Escherichia coli BL21 using [3H]-cGMP as substrate preincubated for 15 mins followed by snake venom 5'-nucleotidase and further incubated for 10 mins by scintillation counting method |
| Affinity data for this assay | |
|---|---|
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