| Assay Method Information | |
| | ChEMBL_2545325 |
| Description: | Inhibition of recombinant human N-terminal His6/Flag-tagged TEV-protease cleavage site fused JMJD3 1637-1675 deletion mutant (1141 to 1682 residues) expressed in baculovirus infected Sf9 insect cells using biotinylated H3K27(14-34)me3 as substrate preincubated for 15 mins followed by substrate addition and measured after 5 mins in presence of alpha-KG, FAS and L-ascorbic acid by Alphascreen assay |
| Affinity data for this assay | |
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