| Assay Method Information | |
| | ChEMBL_1515853 (CHEMBL3614716) |
| Description: | Inhibition of FTO (unknown origin) (31 to 505 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in 15-mer ssRNA (5'-CUUGUCA(m6A)CAGCAGA-3') substrate demethylation by measuring demethylation ratio at 80 uM incubated for 0.5 hrs at room temperature by LC/MS method |
| Affinity data for this assay | |
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