| Assay Method Information | |
| | ChEMBL_1624961 (CHEMBL3867373) |
| Description: | Inhibition of recombinant human DHFR expressed in Escherichia coli preincubated for 15 mins followed by addition of DHF as substrate and NADPH measured after 60 mins by resazurin/diaphorase coupled assay |
| Affinity data for this assay | |
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