| Assay Method Information | |
| | NRas G12D Surface Plasmon Resonance (SPR) Binding Assay |
| Description: | The assay was run at 25° C. with a data collection rate of 10 Hz. All samples were flowed over both a reference surface and an active surface. Samples were injected for 30 s, with a dissociation time length of 120 s, at a flow rate of 100 μL/min and followed by an extra wash with 50% DMSO and a carry-over control injected at 40 μL/min for 30 s. Ten startup cycles were performed at the beginning of the assay. Solvent correction samples were injected once in the middle of the assay, and once at the end. A control sample of 10 μM control compound was injected between two negative control injections (1.0× running buffer containing 3% DMSO) every thirty cycles, to periodically assess NRAS surface stability. Assay was allowed to run for approximately 20-24 hours due to loss of activity of the protein surface over time. Data was analyzed using the Biacore™ S200 Evaluation Software, and all data was fit using a 1:1 kinetic binding model or steady-state fitting, as appropriate. |
| Affinity data for this assay | |
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