Assay Method Information | |
| FRET-Based Assay |
Description: | The protease activity of the full-length NS3-NS4a and the protease domain were measured using a FRET-based assay using a peptide substrate derived from the NS4A/B cleavage site and labeled at one end with a quencher (QXL520) and at the other with a fluorophore (5-FAMsp; Anaspec). |
Affinity data for this assay | |
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