Assay Method Information

Assay Name:  Fluorescence Polarization Assay
Description:  Compounds were tested for binding to Src SH2 using a fluorescence polarization binding assay according to methods previously described. IC50 values were assigned to individual compounds according to their competitive binding affinity versus a high-affinity peptide probe, fluorescein-pTyr-pTyr-pTyr-Ile-Glu-NH2.
Affinity data for this assay
 

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