| Assay Method Information | |
| | EC50 Test |
| Description: | Tet inducible human DDR1 over-expressing U2OS was pre-treated by media containing each of the compound for 1 hour and treated by changing the media to the EC50 test media containing 10ug/ml collagen and compound for 2 hours. Cell were washed with cold PBS three times and lysed with the lysis buffer. The activation of DDR1 was quantified by density using ImageJ to determine EC50 following Western blot using anti-activated human DDR1b(Y513). |
| Affinity data for this assay | |
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