| Assay Method Information | |
| | IC50 Activity Assays |
| Description: | The assays were performed at 25 degrees Celsius in a 100mM sodium phosphate buffer (pH 7.0), with the AR protein amount reaching the Vmax and 0.2mM NADPH. The final reaction volume was 500uL per reaction. All compounds were dissolved in dimethyl sulphoxide, and the corresponding solution was added to the cell and incubated for 5 min at 25 degrees Celsius prior to the addition of the substrate. The reaction was initiated by the addition of 1mM glyceraldehyde, and the decrease in optical density at 340nm was monitored for 3 min at 25 degrees Celsius in a UV-vis spectrophotometer. |
| Affinity data for this assay | |
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