| Assay Method Information | |
| | ITC experiments |
| Description: | ITC experiments were carried out using iTC200 (Microcal LLC, Northampton, MA, USA) with each of the compounds 1-6 and Mcl-1 protein. The heat of reaction was obtained by making 16 injections (2.4 µL each) of the ligand (final concentration in the syringe of 200 µM in 50 mM Tris, 150 mM NaCl, and 5% DMSO, pH 8.0) into the reaction cell that contained Mcl-1 in the same buffer (final protein concentration of 20 µM). The experiments were done at 25°C and at a stirring speed of 500 rpm. For the control experiment, the sample cell was filled with the assay buffer and the compound solution was added, respectively. |
| Affinity data for this assay | |
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