Assay Method Information

Assay Name:  MAO Assay
Description:  The reaction mixture (final volume 200 µL) contained 5 µg/mL MAO-A or MAO-B in a 50 mM sodium phosphate buffer pH 7.4, 1 mM p-tyramine (substrate for MAO-A) or 1 mM benzyl-amine (substrate for MAO-B), 1 U/mL HRP, and 200 µM AR was incubated, in the presence or absence of the inhibitors, at 37 °C for 45 min. The enzyme plus inhibitors were incubated at 37 °C for 20 min followed by the addition of the substrates, HRP and AR. The inhibitors were dissolved in 100% DMSO, and equivalent concentrations of DMSO alone (1-2%) were used as controls. Clorgyline (MAO-A inhibitor) or pargyline (MAO-B inhibitor) at 5 µM concentration was used as positive control for inhibition.
Affinity data for this assay

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