| Assay Method Information | |
| | Urease Inhibition Assay |
| Description: | The assay mixture contained 40 uL of reaction buffer (100 mM urea, 0.01 M K2HPO4, 1 mM EDTA, and 0.01 M LiCl2, pH 8.2), 10 uL of urease (5 U/mL) and 10 uL of test compound. The mixture was incubated in a 96-well plate at 30 uC for 30 min, followed by the addition of equal volumes (40 uL) of phenol reagents (1% w/v phenol and 0.005% w/v sodium nitroprusside) and alkali reagent (0.5% w/v NaOH and 0.1% active chloride NaOCl, and the absorbance was measured. |
| Affinity data for this assay | |
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