Assay Method Information | |
| Steady-State Kinetics Assay |
Description: | Steady state kinetics were performed on an Agilent 8453 diode array spectrophotometer in 10 mM phosphate-buffered saline (pH 7.4). IC50, defined as the inhibitor concentration resulting in a reduction of NCF (100 μM) hydrolysis by 50%, was determined by measurements of initial velocities after 5 min preincubation of enzyme with inhibitor. |
Affinity data for this assay | |
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