| Assay Method Information | |
| | FLIPR Assay |
| Description: | Activation of the mGluR5 receptor expressed in cell lines results in an increase in intracellular calcium concentration. Using calcium sensitive fluorescent dyes and a suited fluorescence plate reader this functional response is detectable and quantifiable. This technique could be used to characterize pharmacological modifications of the mGluR5 receptor.[Ca]i measurements were performed in HEK293 cells stably expressing the full-length human mGlu5a receptor under the control of a tet-regulated promoter. Cells were cultivated in Dulbecco's modified eagle's medium (DMEM) with 10% fetal calf serum, 100 ug/ml HygromycinB, 500 ug/ml G418 and 2 ug/ml Tetracycline in a 37, 95% humidity and 5% CO2 incubator. Confluent cell cultures were split on a bi-weekly schedule.72 hours prior to the assay run mGluR5a expression was induced by replacing the culture medium by DMEM with 10% fetal calf serum without antibiotics. |
| Affinity data for this assay | |
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