| Assay Method Information | |
| | MAO Inhibition Assay |
| Description: | The protocol for measuring IC50 values for the inhibition of MAO-A and MAO-B has been reported in detail in a recent publication (26). The recombinant human MAO enzymes were used, and kynuramine served as non-selective MAO substrate. The MAOs oxidize kynuramine to ultimately yield a fluorescent metabolite, 4-hydroxyquinoline, which was measured by fluorescence spectrophotometry (kex = 310; kem = 400 nm). |
| Affinity data for this assay | |
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