| Assay Method Information | |
| | AChE Inhibition Assay |
| Description: | AChE inhibitory activity was measured with a Molecular Devices Spectra Max Plus 384 spectrophotometer (CA, USA) based on Ellman's method with slight modification. For each assay, 200 µl of the assay medium containing 100 µl of 0.1 M phosphate buffer (pH 8), 20 µl of 3.3 mM 5,50-dithiobis(2-nitrobenzoic acid) in 0.1 M phosphate buffer (pH 7) containing 6 mM NaHCO3, 20 µl of the test compoundsat different concentrations in methanol, and 40 µl of 0.1 unit/ml AChE in phosphate buffer (pH 8) were incubated at 37 °C for 20 min. Subsequently, 20 µl of 5 mM acetylthiocholine iodide in water was added, and the absorbance was measured at 412 nm every 20 s for 3 min. The rate of each reaction was calculated by Microplate Manager software. The blank percentage of inhibition of the tested compounds was determined by comparing the rate of the sample to the blank. The IC50 value was determined by non-linear regression of the log inhibitor concentration versus the percentage of inhibition using G |
| Affinity data for this assay | |
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