Assay Method Information

Assay Name:  In vitro Assay of alpha-Glucosidasee Inhibitory Activity
Description:  The test compounds were dissolved in DMSO to prepare the required distributing concentration. α-Glucosidase inhibitory activity was assayed by using 50 mM phosphate buffer (pH 6.8) at 37 °C. The enzymatic reaction mixture composed of 20 µL α-glucosidase (0.2 U), 10 µL of various concentrations of test compounds and 140 µL of phosphate buffer was incubated at 37 °C for 10 min. Then 0.5 mM p-nitrophenyl α-D-glucopyranoside (30 µL) was added to the mixture as a substrate. After further incubation at 37 °C for 30 min. The absorbance was measured spectrophotometrically at 405 nm. The sample solution was replaced by DMSO as a control. Acarbose was used as a positive control. All experiments were carried out in triplicates.
Affinity data for this assay
 

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