| Assay Method Information | |
| | AChE Inhibitory Assay |
| Description: | In brief, reaction mixture composed of 10 無 of test sample of five different concentrations, 145 無 phosphate buffer 200 mM (pH 7.7), 80 無 of dithiobisnitrobenzoic acid (DTNB) (18.5 mg of DTNB dissolved in 10 mL phosphate buffer pH 7.7), and 10 無 of enzyme (0.4 U/mL). The mixture was incubated at 25 蚓 for 5 min. Subsequently, the enzymatic reaction was initiated by addition of 15 無 of 1 mM of acetylthiocholine iodide or butyrylthiocholine iodide (according to the respective enzyme), and the mixture was again incubated for 5 min at 25 蚓. The rate of absorbance change was measured at 412 nm for 6 min using a microplate reader (Bio-Rad 680, Germany). Galantamine was applied as positive drug. |
| Affinity data for this assay | |
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