| Assay Method Information | |
| | FABP1 Fluorescent Ligand Displacement Assay |
| Description: | The following fluorescent ligand displacement assays at 24 °C were used to further confirm and/or determine if the cytosolic lipidic ligand "chaperone" proteins FABP1, SCP2, and ACBP also bound nonfluorescent NAEs, 2-MGs, phytocannabinoids, synthetic cannabinoids, or inhibitors: (i) NBD-stearic aciddisplacement, (ii) ANS displacement, (iii) DAUDA displacement, (iv) cis-parinaroyl-CoA displacement, and (v) NBDAEA displacement analogous to NBD-stearic acid displacement. Data were corrected for the following blanks/controls: protein only, fluorescent ligand only, fluorescent ligand with anincreasing amount of nonfluorescent ligand, and photobleaching. |
| Affinity data for this assay | |
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