Assay Method Information

Assay Name:  FABP1 Fluorescent Ligand Displacement Assay
Description:  The following fluorescent ligand displacement assays at 24 C were used to further confirm and/or determine if the cytosolic lipidic ligand "chaperone" proteins FABP1, SCP2, and ACBP also bound nonfluorescent NAEs, 2-MGs, phytocannabinoids, synthetic cannabinoids, or inhibitors: (i) NBD-stearic aciddisplacement, (ii) ANS displacement, (iii) DAUDA displacement, (iv) cis-parinaroyl-CoA displacement, and (v) NBDAEA displacement analogous to NBD-stearic acid displacement. Data were corrected for the following blanks/controls: protein only, fluorescent ligand only, fluorescent ligand with anincreasing amount of nonfluorescent ligand, and photobleaching.
Affinity data for this assay

If you find an error in this entry please send us an E-mail