| Assay Method Information | |
| | Enzymatic Assay |
| Description: | Furin activity was measured in triplicate in wells of a 96-well plate in 0.2 ml 50 mM HEPES, pH 7.5, containing 1 mM CaCl2, 0.005% Brij-35 and 20% glycerol. Pyr-RTKR-AMC (25 μM) was used as a substrate. The concentration of furin in the reactions was 50 nM. The steady-state rate of substrate hydrolysis was monitored continuously (λex=360 nm and λem=465 nm) at 37° C. using a Spectramax Gemini EM fluorescent spectrophotometer (Molecular Devices). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |