Assay Method Information

Assay Name:  Homogeneous Time-resolved Fluorescence (HTRF) Assay
Description:  The assay was using baculovirus-expressed recombinant protein kinase purified as the intracellular domain fused by GST tag, interacting with biotinylated peptide substrate. HTRF is based on the proximity of europium cryptate (donor fluorophore)-labeled antiphosphotyrosine and streptavidin labeled with APC(allophycocyanin), which have been brought together by a binding reaction. When the two entities come into close proximity and upon excitation, energy transfer occurs and APC re-emits at 665 nm.
Affinity data for this assay
 

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