| Assay Method Information | |
| | POX Assay |
| Description: | POX reactions were conducted in 2 ml of filtered and degassed 50 mM Tris-HCl, pH 8.0, containing 50 mM NaCl, 0.1 mM H2O2, 4.5 mM guaiacol, and the indicated concentrations of heme essentially as described previously [Yuan et al., Proc. Natl. Acad. Sci. USA., 103:6142-6147; Mbonye et al., J. Biol. Chem., 281:35770-35778]. |
| Affinity data for this assay | |
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