| Assay Method Information | |
| | Oatp1d1 Transport Assay |
| Description: | In the inhibition experiments, the cells were preincubated for 20 s with test compounds, followed by a 5-min incubation with [3H]E3S (5 nM) or 30-min incubation with LY (5 μM). For the interactors that showed uptake inhibition above 50%, Ki values were determined. For the purpose of distinguishing the type of interaction with chosen compounds, the shift in Km and Vmax values for LY was determined in the presence of a target compound (at a concentration equal to the Ki value of a compound), at varying LY concentrations (8 points) and after 15 min of incubation, which was within the linear range of the LY transport rate. |
| Affinity data for this assay | |
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