| Assay Method Information | |
| | Fluorescence-based Affinity Assay |
| Description: | The fluorescence emission spectra of tryptophan residues were used to monitor the changes in local chemical environment of protein upon ligand binding [Zhu et al., Bioorg. Chem., 68:259-265; Tyler et al., Biochemistry, 49:951-957]. The protein concentration was kept at a constant. Changes in fluorescence were measured upon titration of tested compound to protein solution. Fluorescence was measured by a PE fluorescence spectrophotometer. |
| Affinity data for this assay | |
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