| Assay Method Information | |
| | Thymidine Phosphorylase Inhibition Assay |
| Description: | TP inhibition assay was performed spectrophotometrically in 96-well plate. The method of Bera et al. was followed with slight modifications [Bera et al., Chem. Biol. Drug Des., 82:351-360]. The total reaction volume was 200 μL. 20 μL of TP enzyme (0.058 unit/well) was incubated with 10 μL of test compounds (0.5 mM) for 10 min at 30 C. Thereafter, thymidine (20 μL; 1.5 mM) was added and changes in absorbance were observed for 10 min at 290 nm in 96-well ELISA plate reader (Spectramax, Molecular Devices, CA, USA). Every experiment was run in triplicate. 7-Deazaxanthine was used as a reference. |
| Affinity data for this assay | |
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