| Assay Method Information | |
| | Sialidase Assay |
| Description: | Briefly, the reaction mixtures containing the substrates and up to 100 nM sialidase were incubated at 37°C and stopped by the addition of 0.5 M Na2CO3 pH 9.0 for the colorimetric assay or 0.25 M glycine pH 10.0 for the fluorimetric assay. Released p-NP was detected spectrophometrically at 405 nm. The fluorescenceassociated with the release of 4-MU was measured at the excitation wavelength of 365 nm and an emission wavelength of 445 nm. These measurements were performed using an EnVision microplate reader (Perkin Elmer, Waltham, Mass.). |
| Affinity data for this assay | |
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