| Assay Method Information | |
| | DNA-PK Enzyme Inhibitory Assay |
| Description: | The assays of DNA-PK were performed by Reaction Biology Corporation, One Great Valley Parkway, Suite 2 Malvern, PA 19355 USA. All Compounds were dissolved in DMSO (negative control solvent) and tested for their ability to inhibit human DNA-PK enzyme activity. All synthesized compounds were tested in a 10-dose IC50 profile with 4-fold serial dilutions starting at concentration 100 μM. The control compound, LY294002 was tested in a 10-dose IC50 profile with 3-fold serial dilutions starting at 10 μM concentration. Reactions were carried out using 20 μM peptide substrate [EPPLSQEAFADLWKK], 10 μg/ml DNA and 10 μM ATP using the HTRF assay format. |
| Affinity data for this assay | |
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