| Assay Method Information | |
| | Heme Binding Assay |
| Description: | Heme binding was tracked by difference spectroscopy in the Soret region of the UV−visible spectrum. Successive aliquots of 0.5 mM hemin in 0.1 M NaOH were added to both the sample cuvette, which contained 10 μM apo-HutZ, and the referencecuvette. Spectra were recorded 3 min after the addition of each heme aliquot. |
| Affinity data for this assay | |
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