| Assay Method Information | |
| | COX Inhibition Assay |
| Description: | Selected 1,3,4-oxadiazole derivatives (4a, 4b, 4c, 4e, 6b, 6e, 8b) were tested for their ability to inhibit COX-1 and COX-2 using a COX-(ovine) inhibitor screening kit (Catalogue No. 560101; Cayman Chemical, Ann Arbor, MI). Stock solutions of test compounds were dissolved in a minimum volume of dimethyl sulphoxide (DMSO) toobtain 0.001, 0.01, 0.1, 1, 10, 100, and 500 μM in a final volume of 1 mL. In brief, haematin reconstituted purified COX-1 and COX-2 enzymes in a reaction buffer containing Tris-hydrochloric acid (HCl) (0.1 M, pH 8.0), 5 mM ethylenediaminetetraacetic acid (EDTA), and 2 mM phenol were pre-incubated at room temperature for 1 h with test compounds followed by the addition of AA (100 μM)for 2 min at 37°C. Reactions were terminated by adding 50 μL of 1 M HCl followed by the addition of 100 μL of stannous chloride. |
| Affinity data for this assay | |
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