| Assay Method Information | |
| | Enzymatic Assay |
| Description: | Enzyme solutions were prepared in gelatine solution (1%), at a concentration of 2.5 units/mL. AChE or BChE solution (50 μL) and compound solution (50 μL), which is prepared in 2% DMSO at a concentration range of 10^−1-10^−6 mM, were added to 3.0 mL phosphate buffer (pH8 ± 0.1) and incubated at 25°C for 5 min. The reaction was started by adding (DTNB) (50 μL) and ATC (10 μL) to the enzyme-inhibitor mixture. The production of the yellow anion was recorded for 10 min at 412 nm. As a control, an identical solution of the enzyme without the inhibitor was processed following the same protocol. The blank reading contained 3.0 mL buffer, 50 μL 2% DMSO, 50 μL DTNB and 10 μL substrate. All processes were assayed in triplicate. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |