| Assay Method Information | |
| | Hydratase and Esterase Activity Assays |
| Description: | The effect of novel sulfonamides 28-33 on HCA isozyme activity was determined colorimetrically using CO2-hydration method of Wilbur and Anderson29 described previously [G l in et al., Biol. Pharm. Bull., 27:613-16]. Esterase activity was determined according to the method described by Verpoorte et al [Verpoorte et al., J. Biol. Chem., 242:4221-9]. |
| Affinity data for this assay | |
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