| Assay Method Information | |
| | Stimultaneous Relaxation Assay |
| Description: | In simultaneous relaxation assay, CPT concentrations were 50 and 100 μM, whereas concentration of compounds were 50, 100 and 200 μM. Relaxation assay was carried out as described previously with LdTOPILS serially diluted in the relaxation buffer (25mM Tris-HCl pH 7.5, 5% glycerol, 0.5mM DTT, 10mM MgCl2, 2.5mM EDTA and 150 mg/ml BSA) supercoiled pBluescript (SK ) DNA and 50mM KCl. The amount of supercoiled monomer DNA band fluorescence after ethidium bromide (EtBr) (0.5 mg/ml) staining was quantitated by using GEL Doc 2000 from Biorad (Hercules, CA, USA). |
| Affinity data for this assay | |
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