| Assay Method Information | |
| | Competition Binding Assay |
| Description: | The inhibition constant (Ki) from a GPR6 competition binding assay and the IC50 values from a hERG functional assay for the compound of Formula 1 (Example 1) and for Compound A. As described above, Ki for each compound was obtained using a competition binding assay which employed a filtration-based format utilizing membranes prepared from CHO-K1 cells expressing human GPR6 cDNA; IC50 for each compound was obtained using a hERG functional assay which employed an automated whole cell patch-clamp system utilizing CHO-K1 cells transfected with human hERG cDNA. |
| Affinity data for this assay | |
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