| Assay Method Information | |
| | AKT Kinase Inhibiting Activity |
| Description: | Envision model plate reader (Molecular Devices)White 384-well plate (Thermo, Art. No. #264706)Main reagents included in an HTRF kinEASE TK kit (Cisbio, Art. No. #62TKOPEC)TK-biotin substrateStreptavidin-XL665Europium-labeled tyrosine kinase substrate antibody5× enzyme reaction bufferSEBHTRF assay bufferAKT1 (Cama, Art. No. #01-101)AKT2 (Cama, Art. No. #01-102)AKT3 (Invitrogen, Art. No. #PV3185)10 mM ATP (Invitrogen, Art. No. #PV3227)1 M DTT (Sigma, Art. No. #D5545)1 M MgCl2 (Sigma, Art. No. #M8266). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |