Assay Method Information | |
| Biochemical Assay |
Description: | The primary biochemical assay uses a fluorometric assay for monitoring the replacement of bound nucleotide with a fluorogenic GTP derivative bearing a BODIPY FL moiety, allowing for the identification of compounds that can inhibit this exchange. In certain embodiments, the methods disclosed herein may exploit the reduction of intramolecular fluorescence quenching of the modified nucleotide upon its binding to the target protein. The concentration of Arf6 = 20nM. |
Affinity data for this assay | |
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