| Assay Method Information | |
| | Activity assay for influenza virus neuraminidase |
| Description: | Experimental materials:NA (neuraminidase) solution: allantoic fluid of chicken embryo infected by influenza virus;Enzyme catalyzed reaction system: 330 mmol/L of MES buffer solution (pH 3.5); 200 μmol/L of fluorogenic substrate MUNANA (2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid);4 mmol/L of CaCl2 solution; Stop buffer: 14 mmol/L of NaOH solution (14 mmol/L, 83% ethanol); Oseltamivir: 1 mmol/L;Enzyme activity assay: NA solution (with different diluted concentration) 40 μl; MES (fatty acid methyl ester sulfonate) (330 mmol/L): 10 μl; CaCl2 (4 mmol/L): 10 μl; MUNANA (200 μmol/L): 10 μl; H2O: 30 μl; The above materials were mixed together and incubated for 15 minutes, and 150 μl of the stop buffer was added: EX=355 nm XM=460 nm. |
| Affinity data for this assay | |
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