| Assay Method Information | |
| | TNIK Inhibition Assay |
| Description: | The compounds were studied for function as TNIK inhibitors to generate the data of Table 1. The compound was incubated with 8 mM MOPS at pH 7.0, 0.2 mM EDTA, 250 μM TNIK control peptide, 10 mM Magnesium acetate, and [γ-33P-APT] (specific activity and concentration as required). The reaction is initiated by the addition of the Mg/ATP mix. After incubation for 40 minutes at room temperature, the reaction is stopped by the addition of phosphoric acid to a concentration of 0.5%. 10 μl of the reaction is then spotted onto a P30 filtermat and washed four time for 4 minutes in 0.425% phosphoric acid and once in methanol prior to drying and scintillation counting. Additional information can be found in Eurofins Kinase Profiler Service Assay Protocols v85.2, which is incorporated herein by specific reference in its entirety. |
| Affinity data for this assay | |
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