Assay Method Information

Assay Name:  ChEMBL_1644823 (CHEMBL3993752)
Description:  Non-competitive inhibition of His6-tagged full length PHGDH (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as reduction in NADH formation using 3-phosphoglycerate as substrate preincubated for 30 mins followed by substrate addition in presence of NAD+ by fluorescence assay
Affinity data for this assay
 

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