| Assay Method Information | |
| | In-Vitro Radioligand Binding Assasy |
| Description: | Example 3: Determination of Ki Value at 5-HT2A ReceptorCompound was tested according to the following procedures.Materials and Methods:Receptor source: Recombinant mammalian cellsRadioligand: [3H]-Ketanserine (47.3 Ci/mmol)Final ligand concentration [1.75 nM]Non-Specific Ligand: 0.1 mM 1-Naphthylpiperazine (1-NP)Reference compound: 1-Naphthylpiperazine (1-NP)Positive control: 1-Naphthylpiperazine (1-NP)Incubation conditions: Reactions were carried out in 67 mM Tris-HCl (pH 7.4) for 1 hour at 37° C. The reaction was terminated by rapid vacuum filtration onto the glass fiber filters. Radioactivity trapped onto the filters was determined and compared to the control values in order to ascertain any interactions of the test compound(s) with the cloned serotonin 5-HT2A binding site. |
| Affinity data for this assay | |
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