| Assay Method Information | |
| | Determination of Affinity of Human D2L Receptors in Competitive Binding Assay Using [3H]spiperone |
| Description: | Aliquoted membrane preparations were incubated with 0.16 nM [3H]spiperone (PerkinElmer) in the presence or absence of test compound in 96-well plates for 120 minutes at 25° C. in an incubation buffer containing 50 mM Tris-HCl, 1.4 mM ascorbic acid, 0.001% BSA, 150 mM NaCl, pH 7.4, with 1% DMSO in a final reaction volume of 222 μL. Non-specific binding (NSB) was determined in the presence of 10 μM haloperidol (Sigma-Aldrich). After incubation, samples were filtered over UniFilter® GF/C plates (PerkinElmer), washed and Microscint-20 scintillation cocktail was added. Radioactivity was determined with a MicroBeta2 microplate counter (PerkinElmer). |
| Affinity data for this assay | |
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