| Assay Method Information | |
| | Inhibitory Activity on Human TRPA1 by Conventional Patch Clamp Assay |
| Description: | Table 3.2: The cells were transferred to the perfusion tank and perfused with extracellular fluid. The intracellular fluid was: 130 mM K-aspartate; 5 mM MgCl2; 5 mM EGTA; 10 mMHEPES; pH 7.2 (KOH titration). The intracellular fluid was stored in a small amount in a −80 degree refrigerator in batches, and thawed on the day of the experiment. The electrode was pulled with PC-10 (Narishige, Japan). Whole-cell patch clamp recording was adopted and noise was filtered at one-fifth of the sampling frequency. |
| Affinity data for this assay | |
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