| Assay Method Information | |
| | CYP Inhibition Assay |
| Description: | In vitro studies in human liver microsomes were run according the standard method. In summary, seven different concentration of the test article or a single concentration of a positive control were co-incubated with a single concentration the probe substrate for each of the CYP450 enzyme in pooled human liver microsomes for 5-10 minutes and then the reactions were terminated by addition of 0.1% formic acid in acetonitrile. The samples were then analyzed by LC-MS/MS for the quantification of the probe substrate left after the reaction and the IC50 values were determined by non-linear regression. The substrates for CYP2C9, CYP2D6, CYP3A4 were diclofenac, dextromethorphan and midazolam/testosterone respectively. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |