| Assay Method Information | |
| | Enzyme Inhibition Microplate Assay |
| Description: | For selected lead compounds from fluorescence-based high-throughput screening, the concentrations of inhibitor that caused 50% inhibition of enzymatic reaction (IC50) were calculated by plotting percent inhibition against the logarithm of inhibitor concentration (at least 6 points), and the mean values of at least 3 experiments with standard deviation less than 15 %. Km and Vmax were calculated using the double-reciprocal Lineweaver-Burk plot of rate versus substrate concentration, the values of Ki was obtained using replots of inhibitor concentration against Km/Vmax and I/Vmax. |
| Affinity data for this assay | |
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