Assay Method Information | |
| PKA/PKC Kinase Assay |
Description: | The kinase assay uses purified recombinant enzyme and a biotinylated peptide as substrate. The biotinylated peptides were immobilized on streptavidin-coated FLASH plates. After reaction, the 33P-phosphopeptide captured on the FLASH plates was measured with a TopCount Packard Instruments gamma counter (Packard Instruments, Boston, MA). IC50s were calculated using the sigmoidal curve fit with the Assay Explorer software (MDL, Los Angeles, CA). |
Affinity data for this assay | |
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