Assay Method Information

Assay Name:  K562 Cell Adhesion Assay (alpha4beta7 Mediated Adhesion/MAdCAM-1)
Description:  M2 anti-FLAG antibody coated 96-well plates were coated with recombinant FLAG-hMAdCAM-1 contained in Dulbecco PBS. The compounds to be tested were added. Stably transfected K562 cells expressing human alpha4beta7 integrin that had been labeled with carboxymethyl fluorescein diacetate succinimidyl ester (CFDA-SE) were added to each well and allowed to adhere for 1 h at room temperature. Plates were washed, and then cells were lysed. The plate was read on a 96-well fluorescent plate reader at 485 nm excitation and 530 nm emission.
Affinity data for this assay
 

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