Assay Method Information | |
| Scintillation Proximity Assay |
Description: | SPA assays were run in displacement mode using 30 nM [3H]-GW3965. Ligands were tested in 12-point dose-response curves starting at 20 uM. IC50 values were determined from dose-response and competition curves, which were generated by nonlinear regression analysis using GraphPad Prism. |
Affinity data for this assay | |
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