| Assay Method Information | |
| | C-MET Kinase Assay |
| Description: | The cMET kinase assay was performed in 384-well Fluotrac 200 HiBase microplates using the HTRF KinEASE assay described above for AXL except that the assay volume was reduced to half. Enzyme concentration was 8 ng/mL of recombinant human baculovirus-expressed cMET while the substrate concentrations were 0.1 μM and 0.02 μM for the biotinylated peptide and ATP, respectively. Instead of the Multidrop Combi, the BioRAPTR FRD microfluidic workstation (Beckman Coulter, Brea, Calif.) was utilized for reagent additions. |
| Affinity data for this assay | |
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